Total RNA-Seq provides transcriptional profiling for both the coding and noncoding regions of the genome. Ribo-zero depletion of rRNA is followed by strand-specific library preparation using the TruSeq Stranded Total RNA Library Preparation Kit.
Sample Requirements: Minimum of 500 ng purified RNA at a concentration of 50 ng/ul. A260/280 > 2. RIN < 8 is acceptable.