Services

Human Islet Procurement
Islet Culture
Islet Flow Cytometry
Islet Hormone Assay
Islet Isolation
Islet Perifusion
Islet RNA Isolation
Islet Transplantation
Static Islet Incubation
Training in Islet Procedures

Islet Isolation

The user provides mice. Mouse islets are then isolated by collagenase digestion. Collagenase P in Hank's buffered saline is directly infused into the pancreas through the bile duct. Groups of two pancreata are then digested in 6.7 mL collagenase P for 6-8 min at 37° using a wrist-action shaker. Depending on mouse strain and genetic modification, islets are either handpicked under microscopic guidance or purified using a histopaque gradient. As part of islet isolation service, the IPA Core provides training for end-users who are interested in islet handling and islet picking.